Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AICDA

Cell type

Cell type Class
Blood
Cell type
CD20+ B cells
NA
NA

Attributes by original data submitter

Sample

source_name
total (CD20+) B cells_AID ChIP
subject status
donors undergoing routine tonsilectomy
donor id
donor 1,2 and 3
tissue
tonsil
cell type
Primary Human B cells
chip antibody
AID (Abcam, ab5197)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from Micrococcal treated and sonicated nuclei and histone-DNA complexes isolated with an antibody. Libraries were prepared according to Illumina's instructions accompanying the CHIP-Seq sample prep kit. Libraries were sequenced on the Genome Analyzer II or HiSEq2000 following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
101763813
Reads aligned (%)
28.7
Duplicates removed (%)
32.0
Number of peaks
1627 (qval < 1E-05)

hg19

Number of total reads
101763813
Reads aligned (%)
27.9
Duplicates removed (%)
34.5
Number of peaks
1590 (qval < 1E-05)

Base call quality data from DBCLS SRA